Jove
Visualize
Contact Us

Related Concept Videos

Coagulation01:06

Coagulation

Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
Colloids03:22

Colloids

Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...
Special Staining Techniques01:13

Special Staining Techniques

Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
Biofilms01:29

Biofilms

Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
Flagella and Motility in Bacteria01:18

Flagella and Motility in Bacteria

Flagella are specialized, thread-like structures that extend from a bacteria's cell envelope. They play a crucial role in motility and chemotaxis. Their structural organization and functioning exemplify sophisticated biological engineering, enabling bacterial survival and adaptability in diverse environments.Structure of the FlagellumA bacterial flagellum consists of three key components: the filament, the hook, and basal body. The filament, a long, helical structure composed of repeating...
Colloidal precipitates01:09

Colloidal precipitates

The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

THE EFFECT OF SERUM UPON THE GERMICIDAL ACTION OF SOAPS.

The Journal of experimental medicine·2009
Same author

THE GERMICIDAL AND HEMOLYTIC ACTION OF alpha-BROM SOAPS.

The Journal of experimental medicine·2009
Same author

THE GERMICIDAL ACTION OF HYDROXY SOAPS.

The Journal of experimental medicine·2009
Same author

THE GERMICIDAL ACTION OF alpha-MERCAPTO AND alpha-DISULFO SOAPS.

The Journal of experimental medicine·2009
Same author

CHANGES IN THE STABILITY AND POTENTIAL OF CELL SUSPENSIONS : I. THE STABILITY AND POTENTIAL OF BACTERIUM COLI.

The Journal of general physiology·2009
Same author

CHANGES IN THE STABILITY AND POTENTIAL OF CELL SUSPENSIONS : II. THE POTENTIAL OF ERYTHROCYTES.

The Journal of general physiology·2009
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 19, 2026

Imaging Flow Cytometry to Study Microbial Autoaggregation
05:19

Imaging Flow Cytometry to Study Microbial Autoaggregation

Published on: September 29, 2023

THE FLOCCULATION OF BACTERIA BY PROTEINS.

A H Eggerth1, M Bellows

  • 1Department of Bacteriology, Hoagland Laboratory, Long Island College Hospital, Brooklyn.

The Journal of General Physiology
|October 30, 2009
PubMed
Summary

Adding proteins to bacterial suspensions influences their stability and aggregation. The protein's isoelectric point is key to predicting how protein concentration affects bacterial flocculation, a phenomenon also seen in other colloidal systems.

Area of Science:

  • Biochemistry
  • Colloid Science
  • Microbiology

Background:

  • Bacterial suspensions are complex colloidal systems.
  • Protein interactions can significantly alter colloidal stability.
  • Understanding protein-adsorption phenomena is crucial in various scientific fields.

Purpose of the Study:

  • To investigate the impact of pure proteins on bacterial suspensions.
  • To determine the relationship between protein concentration, H ion concentration, and bacterial flocculation.
  • To explore if this phenomenon extends to non-bacterial colloidal systems.

Main Methods:

  • Bacterial suspensions were treated with pure proteins at varying H ion concentrations.
  • Flocculation zones were observed and analyzed.

More Related Videos

Determination of the Settling Rate of Clay/Cyanobacterial Floccules
06:00

Determination of the Settling Rate of Clay/Cyanobacterial Floccules

Published on: June 11, 2018

Related Experiment Videos

Last Updated: Jun 19, 2026

Imaging Flow Cytometry to Study Microbial Autoaggregation
05:19

Imaging Flow Cytometry to Study Microbial Autoaggregation

Published on: September 29, 2023

Determination of the Settling Rate of Clay/Cyanobacterial Floccules
06:00

Determination of the Settling Rate of Clay/Cyanobacterial Floccules

Published on: June 11, 2018

  • Similar protein treatments were applied to diverse colloidal suspensions (e.g., gold sols, emulsions, erythrocytes).
  • Main Results:

    • Bacterial flocculation zones are directly related to the isoelectric point of the added protein.
    • Higher protein concentrations lead to agglutination near the protein's isoelectric point.
    • Altered stability and flocculation were observed in various non-bacterial colloidal systems upon protein treatment.

    Conclusions:

    • Protein adsorption significantly modifies the stability of bacterial and other colloidal suspensions.
    • The isoelectric point of proteins is a critical factor in protein-induced colloidal aggregation.
    • The observed effects are consistent across a range of colloidal materials, suggesting a general principle of protein-colloid interaction.