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THE PERMEABILITY OF CELLS FOR OXYGEN AND ITS SIGNIFICANCE FOR THE THEORY OF STIMULATION
1Marine Biological Laboratory, Woods Hole.
Living cells readily absorb oxygen, similar to dead cells, indicating that oxygen
Area of Science:
- Cell biology
- Plant physiology
- Biochemistry
Background:
- Cellular respiration relies on oxygen uptake.
- Understanding oxygen permeability is crucial for cellular function.
- Previous studies suggested oxygen availability limits cellular oxidation.
Purpose of the Study:
- To investigate the permeability of living cells to oxygen.
- To determine if sudden oxygen influx triggers increased cellular oxidation.
- To elucidate the mechanism behind oxygen-dependent oxidations in plant cells.
Main Methods:
- Utilized an indicator method to assess oxygen permeability.
- Compared oxygen uptake in living versus dead plant cells.
- Exposed living plant cells to high oxygen pressures.
Main Results:
- Living cells exhibit oxygen permeability comparable to dead cells.
- Sudden oxygen admission does not directly increase cellular oxidation.
- High oxygen pressures in living cells did not immediately initiate oxidations seen in dead cells.
Conclusions:
- Cellular oxygen permeability is high, but intracellular phase boundaries maintain separation of reactants (chromogen and oxidase) in living cells.
- Increased oxygen concentration can eventually lead to cellular injury.
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