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Related Experiment Video

Updated: Jun 19, 2026

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

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Published on: March 12, 2019

THE EFFECT OF RADIO-ACTIVE RADIATIONS AND X-RAYS ON ENZYMES : I. THE EFFECT OF RADIATIONS FROM RADIUM EMANATION ON

R G Hussey¹, W R Thompson

¹Laboratories of The Memorial Hospital and the Department of Pathology, Cornell University Medical College, New York.

The Journal of General Physiology

|October 30, 2009

Summary

Radiochemical decomposition of trypsin by radium emanation was quantified. Trypsin decomposition is proportional to emanation quantity, irradiation time, and trypsin concentration, with beta radiation being the primary cause.

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Published on: July 3, 2015

Area of Science:

Biochemistry
Radiochemistry
Radiation Biology

Background:

Trypsin is a key enzyme in biological systems.
Understanding its stability under radiation is crucial for various applications.

Purpose of the Study:

To quantitatively study the radiochemical decomposition of trypsin.
To determine the factors influencing trypsin decomposition by radium emanation.

Main Methods:

Quantitative analysis of trypsin decomposition.
Irradiation of trypsin solutions with radium emanation.
Mathematical modeling of experimental results.

Main Results:

Trypsin decomposition is dependent on trypsin concentration.
Decomposition is proportional to the quantity of radium emanation (millicuries) and irradiation time (hours).
Beta radiations are identified as the primary cause of observed decomposition.

Conclusions:

A quantitative relationship for trypsin decomposition by radium emanation was established.
Active or undissociated trypsin appears to be the component affected.
Beta, gamma, and X-rays may induce similar effects in dilute trypsin solutions.