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Related Experiment Video

Updated: Jun 19, 2026

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

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ISOELECTRIC POINTS FOR THE MYCELIUM OF FUNGI.

¹Department of Botany of the University of Missouri, Columbia.

The Journal of General Physiology

|October 30, 2009

Summary

Rhizopus nigricans and Fusarium lycopersici exhibit amphoteric properties, with optimal growth at specific pH levels. Their mycelia influence buffer solutions, indicating complex physiological responses to acidity.

Area of Science:

Mycology
Colloid Chemistry
Biochemistry

Background:

Fungal growth and metabolism are significantly influenced by environmental pH.
Understanding the amphoteric nature of fungal mycelia is crucial for predicting their behavior in various conditions.

Purpose of the Study:

To investigate the amphoteric properties of Rhizopus nigricans and Fusarium lycopersici mycelia.
To determine the optimal pH for the growth of Rhizopus nigricans.
To examine the effect of fungal mycelia on buffer solutions.

Main Methods:

Staining fungal mycelia with acid and basic dyes.
Washing mycelia with buffer mixtures of phosphoric acid and sodium hydroxide.
Culturing fungi on potato dextrose agar and broth with varied pH.

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Measuring colony growth and dry matter production.

Main Results:

Rhizopus nigricans mycelium showed isoelectric points near pH 5.0, behaving as an amphoteric colloid.
Optimal growth of Rhizopus nigricans occurred in a double-maximum curve, with minima around pH 5.2.
Fusarium lycopersici mycelium displayed amphoteric properties with an isoelectric point near pH 5.5.
Rhizopus nigricans mycelium altered buffer solutions towards alkalinity within a pH range of 4.1 to 6.3.

Conclusions:

Both Rhizopus nigricans and Fusarium lycopersici exhibit amphoteric characteristics.
Fungal growth is highly sensitive to pH, with distinct optimal ranges and responses.
Mycelial interactions with buffer solutions suggest active physiological processes influencing pH homeostasis.