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Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis
Published on: September 27, 2019
WAVE LENGTH OF LIGHT AND PHOTIC INHIBITION OF STEREOTROPISM IN TENEBRIO LARVAE
1Zoological Laboratory, Rutgers College, New Brunswick.
Abstract:
A definite intensity of white light is required (about 136 m.c.) to produce negative phototropic orientation of creeping Tenebrio larvae away from contact with a vertical glass surface. This gives a measure of stereotropism in terms of phototropism, or reciprocally. The effectiveness of light for the suppression of stereotropism varies with wave length. It is therefore simple to obtain a measure of the relation between wave length and stimulating efficiency in this case of phototropic orientation. By determinations of the minimal energy required to inhibit stereotropism with different regions of the spectrum, it is found that the maximum effectiveness is sharply localized in the neighborhood of 535micromicro. The curve connecting stimulating efficiency with wave length, while giving a picture of the effective absorption by the photosensory receptors, probably does not permit accurate characterization of the essential photosensitive material.

