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06:47
Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
Published on: January 28, 2021
THE PROTEIN ASSOCIATED WITH HEMOLYSIN IN RABBIT SERUM AND PLASMA
1Department of Bacteriology, College of Physicians and Surgeons, Columbia University, New York.
The Journal of General Physiology
|October 30, 2009
Summary
Hemolysin, a protein in immune serum, can be isolated using dialysis. Fibrinogen affects its activity, and alcohol extraction damages hemolysin while enhancing hemagglutinin.
Area of Science:
- Immunology
- Biochemistry
Background:
- Immune serum contains water-insoluble globulins associated with hemolysin.
- Hemolytic activity is crucial for immune responses but its stability is not fully understood.
Purpose of the Study:
- To investigate the separation of hemolysin-associated globulins from immune serum.
- To determine the influence of fibrinogen on hemolytic activity.
- To compare the stability of hemolytic and agglutinating activities.
Main Methods:
- Separation of globulins by dilution and dialysis at optimal pH.
- Assessing the effect of fibrinogen on hemolytic activity in plasma.
- Alcohol extraction to evaluate the stability of hemolysin and hemagglutinin.
Main Results:
- Water-insoluble globulins associated with hemolysin can be isolated via dilution and dialysis.
- Fibrinogen influences the optimal pH for globulin separation and can depress hemolytic activity.
- Hemolytic activity is more labile than agglutinating activity; alcohol extraction destroys hemolysin but increases hemagglutinin.
Conclusions:
- Hemolysin can be effectively separated from immune serum using physical methods.
- Fibrinogen plays a significant role in modulating hemolytic activity.
- Differential stability of hemolysin and hemagglutinin has implications for understanding immune protein function.
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