Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Dialysis

Dialysis

Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...

Dialysis

Dialysis

Renal failure occurs when the kidneys lose their ability to filter waste products from the blood effectively. It can be classified into two types: acute renal failure (ARF) and chronic renal failure (CRF).
Acute kidney injury develops suddenly and can be caused by pre-renal causes (e.g., hypovolemia, shock), intrinsic renal causes (e.g., acute tubular necrosis), or post-renal causes (e.g., urinary obstruction). In contrast, chronic renal failure progresses gradually over time and is often...

Peritoneal Dialysis I: Introduction and Procedure

Peritoneal Dialysis I: Introduction and Procedure

Peritoneal dialysis (PD) is a procedure that facilitates the exchange of solutes, waste products, electrolytes, and excess fluid between the blood in the peritoneal capillaries and a dialysis solution introduced into the peritoneal cavity.Principles of Peritoneal Dialysis (PD)Diffusion: Waste products such as urea and electrolytes move from high concentrations in the blood to low concentrations in the dialysate across the peritoneal membrane. This mechanism is driven by the concentration...

Hemodialysis II: Procedure and Complications

Hemodialysis II: Procedure and Complications

DialyzersA hemodialysis (HD) dialyzer is a plastic cartridge containing thousands of parallel hollow fibers, which serve as semipermeable membranes. These fibers are typically made from cellulose-based or other synthetic materials. During HD, blood is pumped into the top of the cartridge and distributed among these fibers. Simultaneously, dialysis fluid, known as dialysate, is introduced into the bottom of the cartridge, bathing the outside of the fibers. Across the semipermeable membrane,...

Hemodialysis I: Introduction

Hemodialysis I: Introduction

Hemodialysis (HD) is a medical treatment that artificially removes waste products, excess fluids, and toxins from the blood when the kidneys are no longer able to perform these functions effectively. In this process, blood is filtered through a semipermeable membrane, allowing for the selective removal of waste while preserving necessary components like blood cells and proteins. Hemodialysis is typically performed in patients with end-stage renal disease (ESRD) or severe kidney...

Extracorporeal Removal of Drugs: Peritoneal Dialysis and Hemodialysis

Extracorporeal Removal of Drugs: Peritoneal Dialysis and Hemodialysis

Patients with end-stage renal disease (ESRD) or those experiencing drug overdose often require extracorporeal methods to eliminate accumulated drugs and metabolites. Hemoperfusion, hemofiltration, and dialysis are the primary techniques to rapidly remove harmful substances without disrupting the patient's fluid and electrolyte balance. For those with compromised renal function, dosage adjustments of concurrent medications may be necessary during extracorporeal drug removal.Dialysis is a process...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Conditions favoring the growth of adult skin epithelium in vitro.

The Anatomical record·2010

Same author

Crystalline hexokinase, heterophosphatase; method of isolation and properties.

The Journal of general physiology·2010

Same author

A spectrophotometric method for the measurement of ribonuclease activity.

The Journal of biological chemistry·2010

Same author

Isolation of crystalline hexokinase and other proteins from yeast.

The Journal of general physiology·2010

Same author

Crystalline soybean trypsin inhibitor.

The Journal of general physiology·2010

Same author

An improved method for the crystallization of trypsin.

The Journal of general physiology·2010

Same journal

On the mechanism of hypomagnesemia with treatment-resistant seizures caused by variants of the Na+,K+-ATPase α1 subunit (ATP1A1).

The Journal of general physiology·2026

Same journal

Label-free real-time imaging of mitochondrial matrix volume changes and permeability transition in living cells.

The Journal of general physiology·2026

Same journal

Differential regulation of β1-dependent voltage shifts and kinetic modulation by an extracellular glutamate in NaV1.6 VSDIV.

The Journal of general physiology·2026

Same journal

Mechanistic insights into DCPIB inhibition of VRAC: Electrostatic control and binding plasticity.

The Journal of general physiology·2026

Same journal

An epilepsy-associated KV3.1 potassium channel variant acts via dominant-positive effect.

The Journal of general physiology·2026

Same journal

Regulation of olfactory chloride and cyclic nucleotide-gated channels by cholesterol.

The Journal of general physiology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 19, 2026

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Published on: August 10, 2015

DIALYSIS WITH STIRRING.

M Kunitz¹, H S Simms

¹Laboratories of The Rockefeller Institute for Medical Research, Princeton, N. J.

The Journal of General Physiology

|October 30, 2009

Summary

This summary is machine-generated.

This study presents an improved dialysis method using a rocking machine with marbles or air bubbles for enhanced stirring. This technique efficiently removes salts from protein solutions within 24 to 48 hours.

Related Experiment Videos

Last Updated: Jun 19, 2026

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Published on: August 10, 2015

Area of Science:

Biochemistry
Biophysical Chemistry

Background:

Dialysis is a common technique for purifying biological molecules.
Traditional dialysis can be time-consuming and may lack efficient stirring.

Purpose of the Study:

To develop a more efficient method for purifying substances via dialysis.
To reduce the time required for salt removal from protein solutions.

Main Methods:

Substances were placed in collodion bags with a toy marble or air bubble.
Bags were sealed in glass tubes within a rocking machine.
Distilled water was circulated around the bags at 8 cc/min/bag.

Main Results:

The rolling action of marbles/bubbles induced stirring within the dialysis bags.
Efficient removal of salts from protein solutions was achieved.
Purification was completed within a 24 to 48-hour timeframe.

Conclusions:

The enhanced rocking machine dialysis method significantly improves purification efficiency.
This method offers a faster alternative for removing salts from protein solutions.
The technique is suitable for biochemical and biophysical applications requiring purified proteins.