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Updated: Jun 19, 2026
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Quantitative PCR of T7 Bacteriophage from Biopanning
Published on: September 27, 2018
A METHOD FOR THE QUANTITATIVE DETERMINATION OF BACTERIOPHAGE.
1Laboratories of The Rockefeller Institute for Medical Research, Princeton, N. J.
The Journal of General Physiology
|October 30, 2009
Summary
Researchers found a quantitative link between bacteriophage concentration and the time needed to reduce bacteria. This discovery enables precise bacteriophage quantification with 5% accuracy.
Area of Science:
- Microbiology
- Virology
Background:
- Bacteriophages are viruses that infect bacteria.
- Accurate quantification of bacteriophages is crucial for various applications, including phage therapy and diagnostics.
Purpose of the Study:
- To establish a quantitative relationship between bacteriophage concentration and bacterial reduction time.
- To develop a method for the accurate estimation of bacteriophage.
Main Methods:
- Studied staphylococcus and antistaphylococcus phages.
- Maintained constant total reaction volume.
- Measured time to reach an arbitrary turbidity end-point for a specific bacterial concentration.
Main Results:
- A definite quantitative relationship was identified between phage concentration (C(Phage)) and the time required for bacterial reduction.
- This relationship allows for the quantitative estimation of bacteriophage.
Conclusions:
- The established relationship provides a reliable basis for bacteriophage quantification.
- The developed method offers an accuracy of within +/-5% for bacteriophage estimation.
