Related Experiment Video

Updated: Jun 19, 2026

Estimation of Structural Sensitivity of Intrinsically Disordered Regions in Response to Hyperosmotic Stress in Living Cells Using FRET
05:13

Estimation of Structural Sensitivity of Intrinsically Disordered Regions in Response to Hyperosmotic Stress in Living Cells Using FRET

Published on: January 12, 2024

FURTHER STUDIES ON THE KINETICS OF OSMOSIS IN LIVING CELLS

B Lucké1, H K Hartline, M McCutcheon

  • 1Laboratory of Pathology, School of Medicine, the Johnson Foundation for Medical Physics, University of Pennsylvania, Philadelphia, and the Marine Biological Laboratory, Woods Hole.

The Journal of General Physiology
|October 30, 2009
PubMed

More Related Videos

Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis08:08

Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis

Published on: February 19, 2018

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation15:27

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

Related Concept Videos

Osmosis00:47

Osmosis

Approximately 60% to 95% of the weight of living organisms is attributed to water. Therefore, maintaining appropriate water balance within cells is of paramount importance. Osmosis is the movement of water across a semipermeable membrane, such as a cell’s plasma membrane. In living organisms, water plays a crucial role as a solvent—a molecule that dissolves other molecules.Diffusion Versus OsmosisBoth diffusion and osmosis are types of passive transport—cellular transport that does not require...
Osmosis01:30

Osmosis

Osmosis is the movement of free water molecules through a semipermeable membrane.  The water's concentration gradient across the membrane is inversely proportional to the solutes' concentration. Whereas diffusion transports material across membranes and within cells, osmosis transports only water across a membrane, and the membrane limits the diffusion of solutes in the water. Osmosis is a special case of diffusion.
Water, like other substances, moves from a high concentration of free water...
Osmosis and Osmotic Pressure of Solutions02:40

Osmosis and Osmotic Pressure of Solutions

A number of natural and synthetic materials exhibit selective permeation, meaning that only molecules or ions of a certain size, shape, polarity, charge, and so forth, are capable of passing through (permeating) the material. Biological cell membranes provide elegant examples of selective permeation in nature, while dialysis tubing used to remove metabolic wastes from blood is a more simplistic technological example. Regardless of how they may be fabricated, these materials are generally...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Factors Influencing Microbial Growth: Osmolarity01:28

Factors Influencing Microbial Growth: Osmolarity

Osmolarity is the measure of solute concentration in a solution. It plays a critical role in determining water availability for organisms. Water moves across semipermeable membranes through osmosis, flowing from regions of lower solute concentration (more dilute) to regions of higher solute concentration (more concentrated).In high-solute environments, microbial cells lose water, leading to dehydration and inhibited growth. The extent to which water is available to microbes in such environments...
ATP Driven Pumps I: An Overview01:27

ATP Driven Pumps I: An Overview

ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and are...