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PROTEIN COAGULATION AND ITS REVERSAL : GLOBIN
1Laboratories of The Rockefeller Institute for Medical Research, Princeton, N. J., and the Hospital of The Rockefeller Institute for Medical Research, New York.
The Journal of General Physiology
|October 30, 2009
Summary
Acid acetone treatment denatures globin from hemoglobin, but this process is reversible. Soluble globin can be recovered even after further denaturation, challenging previous theories on native globin separation.
Area of Science:
- Biochemistry
- Protein Chemistry
Background:
- Hemoglobin is a protein crucial for oxygen transport.
- Globin, the protein component of hemoglobin, can undergo denaturation.
- Understanding globin's properties is essential for biochemical research.
Purpose of the Study:
- To investigate the denaturation and coagulation of globin using the acid acetone method.
- To determine the reversibility of acid acetone-induced globin denaturation.
- To re-evaluate existing theories on the separation of native globin from hemoglobin.
Main Methods:
- Preparation of globin from hemoglobin via the acid acetone method.
- Induction of denaturation and coagulation using acid acetone.
- Assessment of solubility and reversibility of denaturation under various conditions, including precipitation with trichloracetic acid and heat treatment.
- Critical analysis of experimental data concerning native globin separation.
Main Results:
- Globin prepared by the acid acetone method is confirmed to be denatured.
- The denaturation and coagulation of globin induced by acid acetone were found to be reversible.
- Soluble globin was successfully obtained from acid acetone-treated globin, even after subsequent precipitation with trichloracetic acid or heating to 100°C.
- Experimental evidence did not support Hill and Holden's claim of separating native globin without intermediate denaturation.
Conclusions:
- The acid acetone method results in reversible globin denaturation.
- Previous theories on the direct separation of native globin require further experimental validation.
- Globin's stability and reversibility of denaturation offer insights into protein chemistry.
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