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Updated: Jun 19, 2026

Lethality Bioassay Using Artemia salina L.
Published on: October 11, 2022
SALT EFFECTS ON EGGS AND NAUPLII OF ARTEMIA SALINA L
1Jacques Loeb Laboratory of Stanford University, Pacific Grove.
Abstract:
Eggs of Artemia salina L., the brine shrimp, are easily obtainable in large quantities. Ecdysis takes place in two stages: (a) extrusion of the inner membrane, and (b) ecdysis of the nauplius from that membrane. The conditions which allow for the former are much more varied than those for the latter. Nauplii form in only solutions of a few sodium salts; and, in Mg, Ca, and Sr salts, potassium is very toxic. The possible environment for the nauplii (1 M total molarity) has been ascertained for chlorides of Na, K, Mg, and Ca. The facts observed account for the peculiar distribution of the organism.
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