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Updated: Jun 19, 2026

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
THE ROLE OF PHOSPHATE IN BIOLOGICAL OXIDATIONS
Abstract:
1. The effect of phosphate on the oxidation of glyceric aldehyde by methylene blue, 1-naphthol 2-sulfonate indophenol, and phenol-indophenol has been studied. 2. At pH 4.77 in a phthalate-buffered medium phosphate does not catalyze the reaction. 3. At pH 7.9 in solutions buffered with borate, carbonate, or phenylalanine marked catalysis by phosphate is observed. The effect is most pronounced in borate. 4. Phosphate catalysis, within the limits studied, is strictly a linear function of the phosphate concentration. 5. The high concentration of HPO(4) (=) and the low concentration of PO(4) ( identical with) relative to that of the substrate virtually demand the conclusion that the PO(4) ( identical with) ion is the active catalytic species.
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