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AC Electrokinetic Phenomena Generated by Microelectrode Structures
Published on: July 28, 2008
ELECTROKINETIC PHENOMENA : IX. ELECTROPHORESIS AND ELECTROOSMOSIS
1Department of Biological Chemistry, College of Physicians and Surgeons, Columbia University, New York.
The Journal of General Physiology
|October 30, 2009
Summary
The ratio of electroosmotic to electrophoretic mobility for purified protein surfaces is approximately -1.00. This finding aligns with previous experiments and recent theoretical and experimental studies.
Area of Science:
- Biophysics
- Physical Chemistry
- Surface Science
Background:
- Previous experimental studies on crude protein surfaces indicated a specific mobility ratio.
- Recent theoretical and experimental advancements have provided new insights into protein surface behavior.
Purpose of the Study:
- To determine the electroosmotic and electrophoretic mobility ratio for purified protein surfaces.
- To verify the consistency of this ratio with existing theories and experimental data.
Main Methods:
- Experimental determination of electroosmotic mobility.
- Experimental determination of electrophoretic mobility.
- Calculation of the ratio between the two mobilities.
Main Results:
- The ratio of electroosmotic mobility to electrophoretic mobility for purified protein surfaces was found to be approximately -1.00.
- This result is in agreement with prior findings for crude protein surfaces.
Conclusions:
- The electroosmotic and electrophoretic mobility ratio of -1.00 is a consistent characteristic of purified protein surfaces.
- This finding supports the validity of current theories and experimental observations in protein surface electrokinetics.
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