Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Weak base amines can inhibit class I MHC-restricted antigen presentation.

M T Michalek1, B Benacerraf, K L Rock

  • 1Department of Pathology, Harvard Medical School, Boston, MA 02115.

Journal of Immunology (Baltimore, Md. : 1950)
|January 15, 1991
PubMed
Summary

Ammonium chloride (NH4Cl) and methylamine (CH3NH2) inhibit antigen presentation for both MHC class I and II pathways. Chloroquine only affects MHC class II presentation, suggesting NH4Cl and CH3NH2 target an early intracellular step.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nobel laureates' letter to President Bush.

The Washington post·2002
Same author

Three-dimensional prenatal diagnosis of frontonasal malformation and unilateral cleft lip/palate.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2002
Same author

Isolated major congenital heart disease.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2001
Same author

Variation in fetal femur length with respect to maternal race.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine·2001
Same author

Isolated polydactyly: prenatal diagnosis and perinatal outcome.

Prenatal diagnosis·2000
Same author

The prognostic significance of hyperextension of the fetal head detected antenatally with ultrasound.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2000

Area of Science:

  • Immunology
  • Molecular Biology
  • Cellular Biology

Background:

  • Antigen presentation by Major Histocompatibility Complex (MHC) molecules is crucial for adaptive immunity.
  • Understanding the intracellular pathways influencing MHC class I and II antigen processing and presentation is vital for immune response modulation.

Purpose of the Study:

  • To investigate the differential effects of NH4Cl, CH3NH2, and chloroquine on MHC class I and II restricted antigen presentation.
  • To elucidate the specific intracellular steps targeted by these compounds in antigen processing.

Main Methods:

  • Utilized OVA-specific T-T hybridomas to assess antigen presentation by B lymphoblastoid antigen-presenting cells (APCs).
  • Administered NH4Cl, CH3NH2, and chloroquine to APCs and evaluated antigen presentation associated with H-2Kb (MHC class I) and I-Ad/b (MHC class II) molecules.

Related Experiment Videos

  • Assessed antigen uptake, MHC molecule regeneration, and de novo protein synthesis in drug-treated APCs.

    • Main Results:

    • NH4Cl and CH3NH2 inhibited both MHC class I and II restricted antigen presentation.
    • Chloroquine significantly inhibited MHC class II but not MHC class I restricted antigen presentation.
    • NH4Cl and CH3NH2 did not affect antigen uptake, MHC class I surface expression, or de novo protein synthesis, indicating an early intracellular inhibition point.

    Conclusions:

    • NH4Cl and CH3NH2 inhibit a critical early intracellular step in antigen presentation common to both MHC class I and II pathways.
    • Chloroquine selectively targets MHC class II antigen presentation, likely affecting later stages of processing or loading.
    • These findings differentiate the mechanisms of action for these compounds on antigen presentation pathways.