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One-step Extraction and Zymographic Analysis of Bacterial Gelatinases
Published on: August 1, 2025
GELATINASE AND THE GATES-GILMAN-COWGILL METHOD OF PEPSIN ESTIMATION
1Department of Physiological Chemistry, Yale University, New Haven.
The Journal of General Physiology
|October 30, 2009
Abstract:
The Gates photographic film method for pepsin estimation as developed by Gilman and Cowgill measures an activity corresponding to that determined by the hemoglobin method of Anson and Mirsky rather than that resulting from the use of the gelatin viscosity technique. Therefore, the presence of gelatinase is not a source of great error in the gelatin film procedure.
