Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

IL-1 gene expression in lymphoid tissues.

L M Duncan1, L S Meegan, E R Unanue

  • 1Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.

Journal of Immunology (Baltimore, Md. : 1950)
|January 15, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The level of peptide-MHC complex determines the susceptibility to autoimmune diabetes: studies in HEL transgenic mice.

European journal of immunology·2001
Same author

Deamidation of asparagine in a major histocompatibility complex-bound peptide affects T cell recognition but does not explain type B reactivity.

The Journal of experimental medicine·2001
Same author

Cutaneous b-cell lymphomas of follicular and marginal zone types: use of Bcl-6, CD10, Bcl-2, and CD21 in differential diagnosis and classification.

The American journal of surgical pathology·2001
Same author

Intracellular antibody neutralizes Listeria growth.

Immunity·2001
Same author

Cutting edge: a single MHC anchor residue alters the conformation of a peptide-MHC complex inducing T cells that survive negative selection.

Journal of immunology (Baltimore, Md. : 1950)·2001
Same author

Quantitation of lysozyme peptides bound to class II MHC molecules indicates very large differences in levels of presentation.

Journal of immunology (Baltimore, Md. : 1950)·2001
Same journal

The scaffolding protein AKAP79/150 shapes innate immune responses to allergen.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Optineurin restrains IL-17-associated neuroinflammation in trigeminal ganglia to preserve sensory function after ocular HSV-1 infection.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Crystal structure and immune single-cell atlas provide insights into the functional divergence of type I IFNs in fish.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Complement C3 deficiency increases the effector and cytotoxic functions of NK cells and suppresses tumor growth.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Increased Nur77 is disconnected from TCR affinity in insulin-specific Tregs.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

FTR85 negatively regulates type I IFN antiviral signaling pathway by promoting K48-linked polyubiquitination of IRF3.

Journal of immunology (Baltimore, Md. : 1950)·2026
See all related articles

Interleukin-1 (IL-1) mRNA expression was investigated in mice. Following immune stimulation, IL-1 mRNA was detected in spleen, lymph node, and thymus macrophages, indicating localized immune responses.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Interleukin-1 (IL-1) is a key cytokine involved in immune responses.
  • Understanding IL-1 mRNA expression provides insights into immune cell activation and function.

Purpose of the Study:

  • To investigate the in vivo expression patterns of Interleukin-1 alpha (IL-1α) and Interleukin-1 beta (IL-1β) mRNA in murine lymphoid tissues.
  • To correlate IL-1 mRNA expression with immune stimulation by bacterial lipopolysaccharide (LPS) and sheep red blood cells (SRBC).

Main Methods:

  • In situ hybridization using specific RNA probes for murine IL-1α and IL-1β.
  • Analysis of frozen sections from spleen, lymph node, and thymus of mice subjected to LPS or SRBC injection.
  • Immunohistochemical staining using the F4/80 macrophage marker.

Related Experiment Videos

Main Results:

  • No IL-1 mRNA was detected in lymphoid tissues of unstimulated mice.
  • LPS and SRBC injection induced IL-1α and IL-1β mRNA expression in splenic macrophages (red pulp and marginal zone).
  • Distinct cellular populations in the periarteriolar lymphoid sheath expressed IL-1β mRNA, independent of lymphocyte or F4/80+ macrophage markers. Scattered macrophages in lymph nodes and thymus also showed expression post-stimulation. LPS-injected spleens revealed IL-1 mRNA in megakaryocytes.

Conclusions:

  • Immune stimulation with LPS or SRBC triggers localized IL-1 mRNA expression in specific splenic macrophage populations and other non-lymphoid cells.
  • The findings highlight the dynamic regulation of IL-1 production in response to different immune stimuli within lymphoid organs.
  • Further research is warranted to elucidate the functional significance of IL-1 expression in these distinct cellular compartments.