BOUND WATER IN MUSCLE
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Updated: Jun 19, 2026

In vivo Micro-circulation Measurement in Skeletal Muscle by Intra-vital Microscopy
Published on: May 28, 2007
1Food Investigation Board of the Department of Scientific and Industrial Research, and Low Temperature Research Station, Cambridge, England.
Abstract:
1. The amount of free unfrozen water, i.e. water acting as normal solvent, in frog's muscle at temperatures below the initial freezing-point has been calculated from the vapour pressure isotherm of the muscle. 2. Significant amounts of free water are present at -20 degrees C. The total amount of unfrozen water at -20 degrees C. cannot, therefore, be taken as a measure of the bound water in muscle. 3. The calculated values of free water, when compared with experimentally determined values of total unfrozen water, indicate that the amount of bound water in muscle at various temperatures is small. 4. A temperature considerably below -20 degrees C., roughly between -40 degrees and -60 degrees C., is required to freeze completely the free water in muscle.
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