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High-Throughput Metabolic Profiling for Model Refinements of Microalgae

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TEMPERATURE CHARACTERISTICS FOR THE METABOLISM OF CHLORELLA : II. THE RATE OF RESPIRATION OF CULTURES OF CHLORELLA

C S French¹, H I Kohn, P S Tang

¹Laboratory of General Physiology, Harvard University, Cambridge.

The Journal of General Physiology

|October 30, 2009

Summary

The respiration of green alga Chlorella pyrenoidosa declines over time, utilizing two substances with different respiratory quotients. Temperature affects these processes, indicating distinct metabolic pathways.

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Area of Science:

* Plant Physiology
* Algal Metabolism
* Biochemistry

Background:

* Understanding algal respiration is crucial for aquatic ecosystems.
* Chlorella pyrenoidosa is a common freshwater green alga.
* Respiration rates can be influenced by environmental factors like time and temperature.

Purpose of the Study:

* To investigate the dark respiration of Chlorella pyrenoidosa.
* To determine the effect of time and temperature on respiration.
* To identify the substrates involved in algal respiration.

Main Methods:

* Measurement of oxygen consumption and carbon dioxide production in Chlorella pyrenoidosa.
* Experiments conducted in Knop's solution in the dark.
* Respiration rates analyzed over time and varying temperatures.

Main Results:

* Respiration rates decreased over approximately 25 hours to a stable level.
* Two distinct respiratory substrates (A and B) were proposed with respiratory quotients of 1 and 0.65.
* Temperature characteristics (Arrhenius activation energies) were calculated for the oxidation of both substrates.

Conclusions:

* Chlorella pyrenoidosa respiration involves at least two distinct metabolic pathways.
* The identified substrates and their temperature dependencies provide insight into algal respiratory mechanisms.
* Further research can explore the specific identities of substrates A and B.