Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoreceptors and Visual Pathways01:22

Photoreceptors and Visual Pathways

At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category, whereas...
Anatomy of the Eyeball01:20

Anatomy of the Eyeball

The eye is a spherical, hollow structure composed of three tissue layers. The outer layer — the fibrous tunic, comprises the sclera — a white structure — and the cornea, which is transparent. The sclera encompasses some of the ocular surface, most of which is not visible. However, the 'white of the eye' is distinctively visible in humans compared to other species. The cornea, a clear covering at the front of the eye, enables light penetration. The eye's middle layer, the vascular tunic,...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Visual System01:26

Visual System

Light enters the eye through the cornea, a transparent, dome-shaped surface covering the surface of the eyeball that helps to direct and focus incoming light. This light is then channeled toward the pupil, an adjustable opening whose size is controlled by the iris. The iris, a pigmented muscle, regulates the amount of light entering the eye by contracting or dilating the pupil, thereby ensuring optimal light levels for clear vision.
Once through the pupil, the light passes through the lens, a...
Vision01:24

Vision

Vision is the result of light being detected and transduced into neural signals by the retina of the eye. This information is then further analyzed and interpreted by the brain. First, light enters the front of the eye and is focused by the cornea and lens onto the retina—a thin sheet of neural tissue lining the back of the eye. Because of refraction through the convex lens of the eye, images are projected onto the retina upside-down and reversed.
Depth Perception and Spatial Vision01:15

Depth Perception and Spatial Vision

Depth perception is the ability to perceive objects three-dimensionally. It relies on two types of cues: binocular and monocular. Binocular cues depend on the combination of images from both eyes and how the eyes work together. Since the eyes are in slightly different positions, each eye captures a slightly different image. This disparity between images, known as binocular disparity, helps the brain interpret depth. When the brain compares these images, it determines the distance to an object.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

INTERMITTENT STIMULATION BY LIGHT : II. THE MEASUREMENT OF CRITICAL FUSION FREQUENCY FOR THE HUMAN EYE.

The Journal of general physiology·2009
Same author

INTERMITTENT STIMULATION BY LIGHT : V. THE RELATION BETWEEN INTENSITY AND CRITICAL FREQUENCY FOR DIFFERENT PARTS OF THE SPECTRUM.

The Journal of general physiology·2009
Same author

THE COLOR VISION OF DICHROMATS : I. WAVELENGTH DISCRIMINATION, BRIGHTNESS DISTRIBUTION, AND COLOR MIXTURE.

The Journal of general physiology·2009
Same author

THE COLOR VISION OF DICHROMATS : II. SATURATION AS THE BASIS FOR WAVELENGTH DISCRIMINATION AND COLOR MIXTURE.

The Journal of general physiology·2009
Same author

VISUAL ACUITY AND ILLUMINATION IN DIFFERENT SPECTRAL REGIONS.

The Journal of general physiology·2009
Same author

ENERGY, QUANTA, AND VISION.

The Journal of general physiology·2009

Related Experiment Video

Updated: Jun 19, 2026

Measuring the Behavioral Effects of Intraocular Scatter
05:10

Measuring the Behavioral Effects of Intraocular Scatter

Published on: February 18, 2021

THE RELATION BETWEEN VISUAL ACUITY AND ILLUMINATION.

S Shlaer1

  • 1Laboratory of Biophysics, Columbia University, New York.

The Journal of General Physiology
|October 30, 2009
PubMed
Summary
This summary is machine-generated.

This study investigates how light intensity affects human visual acuity using a novel apparatus. Findings reveal distinct rod and cone system contributions to vision, with detail perception depending on distance, not area.

More Related Videos

The Optokinetic Response as a Quantitative Measure of Visual Acuity in Zebrafish
04:56

The Optokinetic Response as a Quantitative Measure of Visual Acuity in Zebrafish

Published on: October 9, 2013

Binocular Dynamic Visual Acuity in Eyeglass-Corrected Myopic Patients
07:06

Binocular Dynamic Visual Acuity in Eyeglass-Corrected Myopic Patients

Published on: March 29, 2022

Related Experiment Videos

Last Updated: Jun 19, 2026

Measuring the Behavioral Effects of Intraocular Scatter
05:10

Measuring the Behavioral Effects of Intraocular Scatter

Published on: February 18, 2021

The Optokinetic Response as a Quantitative Measure of Visual Acuity in Zebrafish
04:56

The Optokinetic Response as a Quantitative Measure of Visual Acuity in Zebrafish

Published on: October 9, 2013

Binocular Dynamic Visual Acuity in Eyeglass-Corrected Myopic Patients
07:06

Binocular Dynamic Visual Acuity in Eyeglass-Corrected Myopic Patients

Published on: March 29, 2022

Area of Science:

  • Ophthalmology and Visual Neuroscience
  • Photobiology
  • Physiological Optics

Background:

  • Visual acuity is crucial for daily tasks and is influenced by light levels.
  • Understanding the roles of rods and cones in vision at different illuminations is fundamental.
  • Previous models of photoreceptor function provide a basis for investigating visual performance.

Purpose of the Study:

  • To develop and utilize an apparatus for precisely measuring visual acuity across a wide range of light intensities.
  • To determine the relationship between illumination and visual acuity using different test objects (broken circle and grating).
  • To elucidate the distinct contributions of rod and cone photoreceptors to visual perception.

Main Methods:

  • An apparatus was designed with a continuously variable test object size and controlled illumination (1:10^10 range).
  • Visual acuity was measured for two observers under varying light conditions using broken circle and grating targets.
  • The limiting factors in visual resolution (pupil diameter, cone size) were analyzed.

Main Results:

  • A threshold at 0.16 visual acuity was observed, separating rod-mediated (below) and cone-mediated (above) vision.
  • Grating targets yielded different acuity results compared to broken circles, particularly at low light levels.
  • Cone-mediated acuity was limited by pupil diameter (<2.3 mm) or cone size (>2.3 mm), aligning with anatomical data.

Conclusions:

  • Detail perception is primarily a function of distance, not area, for both rods and cones.
  • The data support a unified model of photoreceptor function described by Hecht's stationary state equation.
  • The study provides quantitative insights into the functional differences between rod and cone vision.