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Related Experiment Video

Updated: Jun 19, 2026

IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation
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Published on: May 1, 2011

TRANSFORMATION OF SWINE PEPSINOGEN INTO SWINE PEPSIN BY CHICKEN PEPSIN.

R M Herriott1, Q R Bartz, J H Northrop

  • 1Laboratories of The Rockefeller Institute for Medical Research, Princeton, New Jersey, and the Institute of Experimental Biology, University of California, Berkeley, California.

The Journal of General Physiology
|October 30, 2009
PubMed
Summary

Species-specific activation of pepsinogen occurs because the structure determining enzyme specificity is present in the inactive pepsinogen precursor, not the active pepsin enzyme.

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Area of Science:

  • Biochemistry
  • Enzymology
  • Comparative Biology

Background:

  • Pepsin is a key digestive enzyme.
  • Pepsinogen is its inactive precursor.
  • Understanding pepsinogen activation is crucial for enzymology.

Purpose of the Study:

  • To investigate the species specificity of pepsinogen activation.
  • To determine where the structural basis for species specificity resides.

Main Methods:

  • Activation of swine pepsinogen by chicken pepsin.
  • Activation of chicken pepsinogen by swine pepsin.

Main Results:

  • Swine pepsinogen is activated by chicken pepsin to form swine pepsin.
  • Chicken pepsinogen is activated by swine pepsin to form chicken pepsin.

Conclusions:

  • The structure responsible for species specificity is located within the inactive pepsinogen precursor.
  • This finding clarifies the mechanism of species-specific pepsin formation.