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STUDIES ON PITUITARY LACTOGENIC HORMONE : I. ELECTROPHORETIC BEHAVIOR.
1Institute of Experimental Biology, University of California, Berkeley.
The Journal of General Physiology
|October 30, 2009
Summary
This study used electrophoresis to analyze pituitary lactogenic hormone, finding it highly pure. The research determined the hormone's isoelectric point and mobility characteristics.
Area of Science:
- Biochemistry
- Endocrinology
Background:
- Pituitary lactogenic hormone plays a crucial role in various physiological processes.
- Understanding the biophysical properties of this hormone is essential for its therapeutic and research applications.
Purpose of the Study:
- To assess the homogeneity and determine the electrophoretic mobility of pituitary lactogenic hormone.
- To establish the isoelectric point and mobility characteristics of a highly potent lactogenic preparation.
Main Methods:
- Utilized the Tiselius technique of electrophoresis for homogeneity assessment.
- Measured hormone mobility across varying hydrogen ion concentrations using acetate and phosphate buffers.
- Analyzed schlieren photographs to identify distinct protein bands.
Main Results:
- The lactogenic preparation exhibited a single, sharp band in schlieren photographs, signifying high homogeneity.
- The isoelectric point of the hormone was determined to be 5.70.
- The electrophoretic mobility value was found to be 4.5 x 10(-5).
Conclusions:
- The employed pituitary lactogenic hormone preparation is highly homogeneous.
- Electrophoretic analysis provides key insights into the biophysical properties of lactogenic hormone.
- The study discusses discrepancies in mobility compared to previous preparations.
