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Hormones of the Pituitary Gland

Hormones of the Pituitary Gland

The small, pea-sized pituitary gland is located at the base of the brain. It is crucial in regulating various bodily functions, from growth to reproduction. The gland is divided into the anterior lobe and the posterior lobe. The secretory cell clusters in the pars distalis of the anterior pituitary lobe are controlled by hypothalamic regulators and synthesize six primary hormones.
The most abundantly secreted hormone from the anterior lobe is the growth hormone, which controls overall growth by...

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Related Experiment Video

Updated: Jun 19, 2026

Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish

Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish

Published on: August 16, 2018

STUDIES ON PITUITARY LACTOGENIC HORMONE : I. ELECTROPHORETIC BEHAVIOR.

C H Li¹, W R Lyons, H M Evans

¹Institute of Experimental Biology, University of California, Berkeley.

The Journal of General Physiology

|October 30, 2009

Summary

This study used electrophoresis to analyze pituitary lactogenic hormone, finding it highly pure. The research determined the hormone's isoelectric point and mobility characteristics.

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Area of Science:

Biochemistry
Endocrinology

Background:

Pituitary lactogenic hormone plays a crucial role in various physiological processes.
Understanding the biophysical properties of this hormone is essential for its therapeutic and research applications.

Purpose of the Study:

To assess the homogeneity and determine the electrophoretic mobility of pituitary lactogenic hormone.
To establish the isoelectric point and mobility characteristics of a highly potent lactogenic preparation.

Main Methods:

Utilized the Tiselius technique of electrophoresis for homogeneity assessment.
Measured hormone mobility across varying hydrogen ion concentrations using acetate and phosphate buffers.
Analyzed schlieren photographs to identify distinct protein bands.

Main Results:

The lactogenic preparation exhibited a single, sharp band in schlieren photographs, signifying high homogeneity.
The isoelectric point of the hormone was determined to be 5.70.
The electrophoretic mobility value was found to be 4.5 x 10(-5).

Conclusions:

The employed pituitary lactogenic hormone preparation is highly homogeneous.
Electrophoretic analysis provides key insights into the biophysical properties of lactogenic hormone.
The study discusses discrepancies in mobility compared to previous preparations.