Related Experiment Video
Updated: Jun 19, 2026

An Adapted Optical Density-Based Microplate Assay for Characterizing Actinobacteriophage Infection
Published on: June 30, 2023
ADSORPTION OF BACTERIOPHAGE UNDER VARIOUS PHYSIOLOGICAL CONDITIONS OF THE HOST
1William G. Kerckhoff Laboratories of the Biological Sciences, California Institute of Technology, Pasadena.
Abstract:
1. The adsorption rate constant of phage to bacterium is found to change between wide limits, depending on the physiological state of the bacterium. 2. The experiments of Krueger and of Schlesinger on the residual free phage in contact with an excess of bacteria are discussed and the view of Schlesinger, that they represent phage particles with reduced affinity to the bacterial host is supported by experiments. 3. The theory of von Smoluchowski and Schlesinger is compared with the experiments. 4. The implications of these findings for the assay methods currently used are discussed.
Related Concept Videos
Bacteriophages of the Human Virome
Lytic Cycle of Bacteriophages
Lysogenic Cycle of Bacteriophages
DNA Bacteriophages
Viral Replication: Lytic Cycle
Viral Replication: Lysogenic Cycle

