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Updated: Jun 19, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
ENZYMES IN ONTOGENESIS (ORTHOPTERA) : XIII. ACTIVATION OF PROTYROSINASE AND THE OXIDATION OF ASCORBIC ACID
1Zoological Laboratory, State University of Iowa, Iowa City.
Abstract:
1. Protyrosinase from the egg of the grasshopper, Melanoplus differentialis, can be activated by excess sodium oleate or Aerosol. 2. The 3:4 quinone products of the reaction of activated protyrosinase with tyramine or tyrosine will oxidize ascorbic acid to dehydroascorbic acid. 3. The velocity of this latter oxidation of ascorbic acid increases with the amount of tyramine or tyrosine. 4. The oxidation of ascorbic acid by the tyramine-tyrosinase reaction delays the time of appearance of a red color associated with an indole quinone intermediary product in the formation of melanin. 5. Protyrosinase, in itself, and in the presence of tyrosinase substrates does not bring about the oxidation of ascorbic acid. 6. A naturally occurring substrate in a preparation of protyrosinase, sufficient to cause the oxidation of ascorbic acid, can be removed by dialysis against a 0.9 per cent sodium chloride solution. 7. Dialysis against such a solution does not change the properties of protyrosinase; the inactive enzyme must still be activated before it will catalyze the oxidation of tyramine or tyrosine. 8. When the natural substrate, tyrosine, or tyramine is absent, activation of protyrosinase does not result in the oxidation of ascorbic acid.
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