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STUDIES ON PITUITARY LACTOGENIC HORMONE : III. SOLUBILITIES OF SHEEP AND BEEF HORMONES.
1Institute of Experimental Biology and the Department of Anatomy, University of California, Berkeley.
The Journal of General Physiology
|October 30, 2009
Summary
This study determined the solubility of sheep and beef lactogenic hormones. Differences in solubility indicate species specificity, confirming distinct properties between sheep and beef hormones.
Area of Science:
- Biochemistry
- Endocrinology
- Protein Chemistry
Background:
- Lactogenic hormones play crucial roles in mammalian reproduction and lactation.
- Understanding hormone solubility is vital for purification and characterization.
- Species-specific differences in protein structure can affect physicochemical properties.
Purpose of the Study:
- To determine the solubility of sheep pituitary lactogenic hormone.
- To investigate the solubility of beef lactogenic hormone.
- To analyze the salting-out effect of NaCl on both hormones and assess species specificity.
Main Methods:
- Solubility determination of sheep lactogenic hormone in 0.302 M NaCl at pH 2.02.
- Solubility determination of beef lactogenic hormone in distilled water at 7-8°C.
- Investigation of the salting-out effect of NaCl on both hormones in acidic solutions.
Main Results:
- Sheep lactogenic hormone exhibited constant solubility, indicating a single component.
- Beef lactogenic hormone also showed constant solubility, suggesting purity.
- Both hormones behaved as pure substances, but displayed differential solubility, highlighting species specificity.
Conclusions:
- The preparations of sheep and beef lactogenic hormones were found to be pure.
- The observed differences in solubility confirm species-specific characteristics of these hormones.
- Solubility studies provide a basis for understanding and differentiating between related protein hormones.
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