PURIFICATION OF THROMBIN
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Updated: Jun 19, 2026

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis
Published on: January 9, 2026
1Laboratories of The Rockefeller Institute for Medical Research, Princeton, New Jersey.
Abstract:
1. Under certain conditions crude prothrombin changes to thrombin without the addition of extraneous activators and in the absence of ionic calcium. 2. Thrombin is soluble in 0.45 saturated ammonium sulfate, whereas crude prothrombin is not. 3. Partially purified thrombin is comparatively stable in concentrated ammonium sulfate solutions at pH 5.2. 4. A method based on the above facts yields a thrombin preparation the specific activity of which is 100 to 175 times the potential specific activity of whole plasma.
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