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Updated: Jun 19, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
THE QUANTITATIVE EFFECT OF X-RAYS ON ASCORBIC ACID IN SIMPLE SOLUTION AND IN MIXTURES OF NATURALLY OCCURRING
1Memorial Hospital, New York.
X-ray exposure causes reactions in ascorbic acid within biological solutions like serum albumin and plasma. However, the reaction is minimal in excised rat muscle tissue.
Area of Science:
- Biochemistry
- Radiation Chemistry
- Cellular Biology
Background:
- Ascorbic acid (Vitamin C) is a crucial antioxidant.
- X-ray radiation can induce chemical reactions in biological molecules.
- Understanding these reactions is vital for radiation biology and medicine.
Purpose of the Study:
- To investigate the x-ray induced reactions of ascorbic acid.
- To compare these reactions in different biological environments.
Main Methods:
- Exposure of ascorbic acid solutions to x-ray radiation.
- Analysis of reactions in simple inorganic solutions, serum albumin solutions, human plasma, and excised rat muscle.
Main Results:
- Ascorbic acid exhibited x-ray induced reactions in the presence of serum albumin and human plasma.
- The observed reaction in excised rat muscle was significantly smaller compared to plasma and albumin solutions.
Conclusions:
- Serum albumin and plasma facilitate x-ray induced reactions of ascorbic acid.
- Ascorbic acid's reactivity to x-rays is diminished within muscle tissue environments.
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