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Lucifer Yellow - A Robust Paracellular Permeability Marker in a Cell Model of the Human Blood-brain Barrier
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Published on: August 19, 2019

Lytic agents, cell permeability, and monolayer penetrability.

M R Salton1

  • 1Department of Microbiology, New York University School of Medicine, New York 10016.

The Journal of General Physiology
|October 30, 2009
PubMed
Summary
This summary is machine-generated.

Lytic agents cause cell lysis by disrupting cell membranes, a process influenced by chemical structure and membrane composition. Understanding these interactions aids in developing targeted lysis strategies for various cell types.

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18:57

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Biophysics

Background:

  • Cell lysis involves membrane disorganization and macromolecule release.
  • Lytic agent interactions with cell membranes are influenced by chemical structure and membrane properties.
  • Erythrocytes serve as a model for studying lysis due to their well-characterized membranes.

Purpose of the Study:

  • Investigate the relationship between chemical structure and hemolysis.
  • Explore the role of surface pressure in cell membrane breakdown.
  • Characterize the dissociation of bacterial membranes by surface-active agents.

Main Methods:

  • Studied surface-active agents' effects on erythrocyte membranes.
  • Examined lysis as an interfacial phenomenon.
  • Analyzed dissociation products of bacterial membranes using ultracentrifugation.

Main Results:

  • Lysis occurs when surface pressures exceed a critical collapse level.
  • Membrane cholesterol and phospholipids may be involved in lysis.
  • Bacterial cell walls influence susceptibility to lysis; removal increases sensitivity.

Conclusions:

  • Surface-active agents dissociate isolated erythrocyte and bacterial membranes.
  • Characterizing membrane proteins and lipids is key to understanding lytic agent specificity.
  • Lysis mechanisms share common principles across different cell types due to conserved membrane composition.