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Published on: November 18, 2022
[Identification and analysis of an actinomycete strain suppressing Clavibacter michiganensis subsp, michiganensis]
Yan Zhang1, Weihong Zhang, Songhong Wang
1College of Plant Protection, Agricultural University of Hebei, Biological Control Center for Plant Disease and Plant Pests of Hebei Province, Baoding 071001, China. geneclone@126.com
Objective:
To identify and analyze bioactive compounds of an actinomycete strain Z-L-22 suppressing Clavibacter michiganensis subsp. michiganensis, the causal agent of bacterial canker of tomato.
Methods:
Morphological, biological and biochemical characterization, chemotaxonomy analysis and 16S rDNA sequences homology analysis were performed to identify the strain Z-L-22. Bioactive compounds were separated and retrieved by thin layer chromatography. Paper chromatography and confirmation tests were used to identify the antibiotic. PCR was carried out using the primers targeted to synthetase of the antibiotic.
Results:
Strain Z-L-22 belonged to Streptomyces sp. and was similar to Streptomyces setonii. Two main bioactive components were isolated by thin layer chromatography, which were all identified as actinomycin. New actinomycin synthetase gene was cloned using the primers designed from actinomycin synthetase conserve domain.
Conclusion:
Strain Z-L-22 was classified as Streptomyces setonii. Actinomycin produced by Streptomyces setonii was first reported.
