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Updated: Jun 19, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
[Isolation and characterization of a marine agarase]
Hui Xie1, Baoqin Han, Wen Dong
1College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. pingguo0526@yahoo.com.cn
Objective:
This study was carried out to isolate and characterize an agarase from a marine bacterium Agarivorans albus QM38.
Methods:
SDS-PAGE grade agarase was obtained from the fermentation broth after removing the bacteria by centrifugation, ammonium sulfate precipitation, DEAE-sepharose fast flow anion exchange chromatography and Sephacryl S-100 gel filtration. Enzyme's molecular weight was determined with SDS-PAGE. The catalysates of the isolated enzyme were determined withmass spectrography.
Results:
Agarase A was isolated. The molecular weight of agarase A was 127.80 kDa. More characterizations of agarase A were studied and the results showed that the optimal reaction condition for agarase A was at 35 degrees C, pH 7.6, and agar concentration of 0.9% (w/v), while most of the metal ions inhibited the activity of it. The catalysates of agarase A were mainly tetrose and hexose.
Conclusion:
Agarase A was purified from the medium. It could hydrolyze jellied agar and yield simple catalysates. Its molecular weight is different from all the agarases reported so far.
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