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Updated: Jun 19, 2026
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
Published on: November 1, 2011
[Reverse genetic system for rabies virus vaccine Evelyn-Rokitnicki-Abelseth strain]
Li Guo1, Na Feng, Songtao Yang
1Graduate School, Jilin Agriculture University, Changchun 130118, China. piaogl110@163.com
Objective:
To establish a reverse genetic system of rabies virus for producing safe and efficient rabies vaccine.
Method:
By reverse genetic and molecular cloning technique, we established two rabies virus rescue systems including (1) help plasmids expressing N, P and L protein, and (2) CMV/T7 and T7 promoter.
Results:
Wild-type rERA-VC was rescued by both systems, and had the same growth kinetics as parental virus. The third generation virus could grow to high titer.
Conclusion:
The established reverse genetic system for rescuing wild-type rERA-VC provides the possibility of producing safe and efficient rabies virus vaccines.
