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[Effects of naringin on proliferation, differentiation and matrix mineralization of MC3T3-E1 cells]
Peihui Ding1, Qi Tang, Lili Chen
1Department of Stomatology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China. phding@163.com
Objective:
To investigate the effect of naringin on the proliferation, differentiation and matrix mineralization of MC3T3-E1 cells in vitro.
Method:
MC3T3-E1 cell lines were taken in vitro model. CCk-8 method was used to observe the proliferation of MC3T3 cells. Lactic acid dehydrogenase cytotoxicity (LDH) test was used to observe the cell toxicity. Bone morphogenetic protein-2 (BMP-2), alkaline phosphatase (ALP) and osteocalcin (OC) were used to observe the cell differentiation. Von kossa calcification staining method was used to observe the cell calcification.
Results:
The high dosages of the naringin could promote the proliferation of MC3T3-E1 cells at both 12 h and 24 h. While, low dosages did not show the same capability. LDH test showed that the cytotoxicity percentages in all six naringin treated groups were quite low. BMP-2 cytoimmunochemistry test showed that the three naringin treated group (10, 1, 0.1 micromol x L(-1)) showed higher brown coloration in cytoplasm than the control group at both 24 h and 48 h. 1, 0.1 micromol x L(-1) naringin raised ALP activity of MC3T3-E1 cells at 48 h (P < 0.05). Meanwhile, 0.1 micromol x L(-1) naringin increased the ALP activity at 72 h (P < 0.05). 10 and 1 micromol x L(-1) naringin increased the capability of MC3T3-E1 cell to synthesize osteocalcin during 8th - 12th dsince adding the medicine (P < 0.05). Naringin did not show the positive effects on cell calcification.
Conclusions:
Naringin could promote proliferation and differentiation of MC3T3-E1 cells.
