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Related Experiment Video

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Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Published on: December 8, 2021

LDHC: the ultimate testis-specific gene.

Erwin Goldberg¹, Edward M Eddy, Chongwen Duan

¹Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208, USA. erv@northwestern.edu

Journal of Andrology

|October 31, 2009

Summary

This summary is machine-generated.

Lactate dehydrogenase C (LDHC) is essential for sperm function and fertilization. This review details the history and critical role of LDHC in male germ cells.

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Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment

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Published on: September 11, 2014

Area of Science:

Biochemistry
Reproductive Biology
Genetics

Background:

Lactate dehydrogenase C (LDHC) was the first identified testis-specific isozyme in male germ cells.
Isozymes are ubiquitous in vertebrates, exhibiting developmental, tissue, and cell-specific regulation.

Purpose of the Study:

To review the historical discovery of LDHC.
To present evidence demonstrating LDHC's requirement for successful fertilization.

Main Methods:

Gel electrophoresis was used for initial detection in human spermatozoa and testes.
Immunohistochemistry localized LDHC in spermatocytes and sperm tails.

Main Results:

LDHC is localized in primary spermatocytes and increases after meiosis.
Final localization is in the principal piece of the sperm tail.

Conclusions:

LDHC is a multifunctional enzyme crucial for sperm function.
LDHC is indispensable for achieving fertilization.