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Related Experiment Video

Updated: Jun 19, 2026

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice
06:56

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

Published on: August 30, 2019

A protocol for islet isolation from mouse pancreas.

Dong-Sheng Li1, Ya-Hong Yuan, Han-Jun Tu

  • 1Hubei Key Laboratory of Embryonic Stem Cell Research, Tai-He Hospital, Yunyang Medical College, Shiyan, Hubei, China.

Nature Protocols
|October 31, 2009
PubMed
Summary
This summary is machine-generated.

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This study presents a reproducible protocol for isolating mouse islets, crucial for diabetes research. The method ensures a good yield of quality islets efficiently, making it accessible for researchers without prior experience.

Area of Science:

  • Endocrinology
  • Cell Biology
  • Diabetes Research

Background:

  • Mouse islets are vital for diabetes mellitus research.
  • High-quality islet isolation is essential for reliable experimental outcomes.
  • Existing protocols may be time-consuming or require specialized expertise.

Purpose of the Study:

  • To describe a practical and reproducible protocol for isolating mouse islets.
  • To provide a time-efficient method for obtaining adequate islet yields.
  • To facilitate islet isolation for researchers with limited experience.

Main Methods:

  • In situ pancreas perfusion with collagenase enzyme.
  • Pancreatic tissue digestion.
  • Islet purification techniques.

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A Simple and Rapid Method for Simultaneous Isolation of Primary Islets and Primary Pancreatic Acinar Cells from Mice
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A Simple and Rapid Method for Simultaneous Isolation of Primary Islets and Primary Pancreatic Acinar Cells from Mice

Published on: January 9, 2026

Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
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Effective Isolation of Functional Islets from Neonatal Mouse Pancreas

Published on: January 6, 2017

Related Experiment Videos

Last Updated: Jun 19, 2026

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice
06:56

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

Published on: August 30, 2019

A Simple and Rapid Method for Simultaneous Isolation of Primary Islets and Primary Pancreatic Acinar Cells from Mice
14:39

A Simple and Rapid Method for Simultaneous Isolation of Primary Islets and Primary Pancreatic Acinar Cells from Mice

Published on: January 9, 2026

Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
07:04

Effective Isolation of Functional Islets from Neonatal Mouse Pancreas

Published on: January 6, 2017

Main Results:

  • The protocol yields a reasonable number of islets.
  • The entire isolation procedure takes 30-45 minutes per mouse.
  • The method is demonstrated to be practicable and reproducible.

Conclusions:

  • This protocol offers an efficient and reliable method for mouse islet isolation.
  • Its simplicity makes it suitable for researchers new to the field.
  • The described technique supports consistent diabetes-related investigations.