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Published on: July 1, 2013
Preliminary proteomic analysis of circulating polymorphonuclear neutrophils from rabbits experiencing scald injury
Pi-hong Zhang1, Li-li Li, Ji-zhang Zeng
1Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China. zphong@yahoo.com.cn
Background:
The aim of this study is to identify the differentially expressed proteins in circulating polymorphonuclear neutrophils (PMN) from scalded bacteremia rabbits infected with Staphylococcus aureus to provide a basis to reveal the pathogenesis of burns and sepsis.
Methods:
Rabbits were subjected to sham burn (A), A + bacterial challenge (B), 30% scald injury (C), or C + bacterial challenge (D). Bacterial challenge was inflicted as an injection of 2.0 x 10(8) cfu S. aureus 18 h after burn procedure. Animals were sacrificed 24 h after burn. PMN were isolated, and the differential proteins in the PMN from these animals were identified by two-dimensional electrophoresis coupled with MALDI-TOF-MS; two proteins were confirmed by Western blotting.
Results:
Twenty-one differential protein spots were found, and seven differential proteins were identified. Among the identified proteins, the expression levels of protein disulfide-isomerase and thiol-specific antioxidant protein were down-regulated in groups C and D, and two protein spots of annexin I were identified, one of which was down-regulated and another up-regulated in groups C and D.
Conclusions:
Preliminary proteome changes in PMN from rabbits experiencing scald injury and S. aureus sepsis were revealed, which possibly play an important role in the inflammation and pathogenesis of sepsis after scald injury.
Insights
Researchers identified key protein changes in polymorphonuclear neutrophils (PMN) of rabbits with burn-induced Staphylococcus aureus sepsis. These findings offer insights into the pathogenesis of severe burn infections and sepsis.
Area of Science:
- Proteomics
- Immunology
- Infectious Diseases
Background:
- Sepsis following severe burns is a major cause of mortality.
- Understanding the host response, particularly in immune cells like polymorphonuclear neutrophils (PMN), is crucial for elucidating sepsis pathogenesis.
- Staphylococcus aureus is a common pathogen in burn wound infections and sepsis.
Purpose of the Study:
- To identify differentially expressed proteins in circulating PMN of rabbits with scald injury and Staphylococcus aureus bacteremia.
- To establish a proteomic basis for understanding the pathogenesis of burn-induced sepsis.
Main Methods:
- Rabbits underwent sham burn, sham burn with bacterial challenge, scald injury, or scald injury with bacterial challenge (Staphylococcus aureus).
- Polymorphonuclear neutrophils (PMN) were isolated from blood.
- Differential protein expression was analyzed using two-dimensional electrophoresis coupled with MALDI-TOF-MS, with Western blotting for confirmation.
Main Results:
- Seven differential proteins were identified among 21 altered protein spots.
- Down-regulation of protein disulfide-isomerase and thiol-specific antioxidant protein was observed in scalded and infected rabbits.
- Annexin I showed differential expression, with one spot down-regulated and another up-regulated in the burn and sepsis groups.
Conclusions:
- Preliminary proteomic alterations in PMN during scald injury and S. aureus sepsis were characterized.
- These identified proteins may play significant roles in the inflammatory response and pathogenesis of sepsis following severe burns.
