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Updated: Jun 19, 2026
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
Published on: November 8, 2011
[Study on the influence factors of human peripheral blood B lymphocyte transformation by Epstein-Barr virus]
Qing Liu1, Yanxia Teng, Yong Niu
1National Institute for Occupational Health and Poison Control, Chinese CDC, Beijing 100050, China.
Objective:
To investigate the influence factors of human peripheral blood B lymphocyte transformation by epstein-barr virus (EBV).
Methods:
EB virus was obtained from B95-8 supernatant and quantitated by quantitative PCR amplification. The concentration of cyclosporine A (CSA) concentration of lymphocytes and different time of adding CSA to culture media were selected to examine the effects on the lymphocyte transformation, and the condition of lymphocyte transformation of worker exposed to trichloroethylene was further confirmed.
Results:
The titer of two virus samples were 5.45 x 10(6) and 1.06 x 10(6) copies/ml. Peripheral blood mononuclear cells (PBMCs) was transformed by using CSA at different concentration and adding time to culture fluid. The concentration of PBMCs being transformed was 2 x 10(6)/ml for normal people, but 1 x 10(6)/ml for worker exposed to trichloroethylene. Cell transformation was confirmed by the gradual increase of cell size and cellular clusters.
Conclusion:
The results suggest the key issue is PBMCs being transformed at a concentration of 2 x 10(6)/ml, rather than using CSA at different concentration and adding time to culture fluid. The PBMCs at concentration of 1 x 10(6)/ml for worker exposed to trichloroethylene were transformed, which may be associated with sensitized status of lymphocytes.
