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[Experiment study of blackcurrant on vascular endothelial cells injury induced by hydrogen peroxide]
1First Teaching Hospital of Xinjiang Medical University, Urumqi 830011, China. xj_lily@126.com
Objective:
To study the effect of Blackcurrant on human umbilical vein endothelial ECV-304 cells injury induced by hydrogen peroxide (H2O2).
Methods:
The H2O2 damaged model was established. The effect of Blackcurrant on injury of ECV-304 activity induced by H2O2 was determined by MTT assay. The levels of MDA, NO, ET, PGI2 and LDH activity in cell homogenate were measured with corresponding. Observed the influence of black currant extracts apoptosis induced the ECV-304 by H2O2 by flow cytometry.
Results:
Blackcurrant could inhibit the hypoxia induced ECV-304 reduction (P < 0.05), decrease LDH activity (P < 0.05), reduce the MDA and ET production (P < 0.05), and increased the contents of nitric oxide (NO) and PGI2 (P < 0.05). The apoptosis rate and total apoptosis rate of blackcurrant extracts groups/positive control group/control group are obvious lower than H2O2 model group (P < 0.05).
Conclusion:
The blackcurrant ethyl acetate extract can protect HUVEC damaged by H2O2, the mechanism maybe related to antioxidant. And it can decrease the apoptosis rate and total apoptosis rate of ECV-304 apoptosis induced by H2O2 in order to protect ECV-304.
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