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[Effect of proliferation, c-met and vascular endothelial growth factor expression in the Eca109/9706 cells by
Yingxin Pei1, Xiaoyan Du, Jinping Li
1Department of Nutrition and Food Hygiene, College of Public Health, Zhengzhou University, Zhengzhou 450001, China. pyx@zzu.edu.cn
Objective:
To explore the effect of cell proliferation, c-met and vascular endothelial growth factor (VEGF) expression by liposomal quercetin in the Eca109/9706 cells induced by liposomal quercetin.
Methods:
The suppressing rate of cultured Eca109/9706 cells was detected by MTT assay. After 48-hour-exposure to liposomal quercetin, the expression and cellular localization of c-met and VEGF in Eca109/9706 cells were examined by using immunohistochemistry assay and Western blotting.
Results:
The suppressing rate of Eca109/9706 cells was in the order of LQ2 group > LQ1 group > nLQ group > control group (P < 0.05). The protein expression of c-met and VEGF was found in Eca109/9706 cells cytoplasma as well as in nuclei by using immunohistochemistry assay. The Immuno-reaction intensity of c-met and VEGF was in the order of control group > nLQ group > LQ1 group > LQ2 group (P <0.05). The western blotting showed the intensity of immunoblotting signals for c-met-IR and VEGF-IR was in the order of control group > nLQ group > LQ1 group > LQ2 group (P < 0.05).
Conclusion:
The liposomal quercetin could suppress the proliferation of culture Eca109/9706 cells, which was about to be related with the suppression high expression of c-met and VEGF.
