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Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis
Published on: December 14, 2015
Cultured skin substitutes: a review
1Department of Surgery, University of Cincinnati College of Medicine, Cincinnati, OH 45267, and Shriners Burns Institute, Cincinnati, OH 45229.
Tissue Engineering
|November 3, 2009
Summary
Engineered skin substitutes offer versatile applications in wound healing and product testing. Future advancements aim for gene therapy integration and standardized assessment protocols for improved efficacy.
Area of Science:
- Tissue engineering
- Dermatology
- Biomaterials science
Background:
- Skin substitutes utilize cultured cells and biopolymers for research and therapeutic applications.
- Epidermal substitutes commonly use keratinocytes, while dermal substitutes employ fibroblasts within biopolymers.
Purpose of the Study:
- To review the current state and future directions of engineered skin substitutes.
- To highlight applications in skin biology, wound treatment, safety testing, and gene therapy delivery.
Main Methods:
- Preclinical models assess cellular behavior and tissue function in vitro and after grafting.
- Clinical considerations involve graft preparation time, vascularization, contamination, fragility, and cost.
Main Results:
- Engineered skin substitutes show potential in preclinical and clinical settings.
- Current efficacy evaluation relies on subjective criteria, with a need for objective, quantitative methods.
Conclusions:
- Noninvasive biophysical instrumentation can enhance objective efficacy assessment.
- Future research will focus on gene therapy applications and establishing international fabrication and assessment standards for engineered skin.
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