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Updated: Jun 19, 2026
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Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Published on: May 16, 2017
Carotenoids found in Bacillus
R Khaneja1, L Perez-Fons, S Fakhry
1School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, UK.
Journal of Applied Microbiology
|November 3, 2009
Summary
Pigmented aerobic spore-forming bacteria, primarily Bacillus species, are widespread in the environment. These bacteria produce yellow, orange, and pink carotenoids, offering enhanced UV protection and potential industrial applications.
Area of Science:
- Microbiology
- Biochemistry
- Environmental Science
Background:
- Pigmented bacteria play roles in various ecosystems.
- Spore-forming bacteria, like Bacillus, are known for their resilience.
- Understanding pigment diversity and function is crucial.
Purpose of the Study:
- To explore the variety of pigmented aerobic spore formers in diverse environments.
- To determine the chemical composition of their pigments.
- To investigate the biosynthetic pathways and functional significance of these pigments.
Main Methods:
- Environmental sampling (soil, seawater, gastrointestinal tract).
- Phylogenetic analysis using 16S rRNA sequencing for species identification.
- Pigment analysis via High-Performance Liquid Chromatography-Photo Diode Array (HPLC-PDA).
Main Results:
- Commonly identified pigments were yellow, orange, and pink carotenoids.
- Isolates were predominantly Bacillus species, including known species like B. marisflavi and B. safensis.
- Three main carotenoids with distinct absorption maxima were identified, suggesting a C30 biosynthetic pathway. A water-soluble carotenoid was also detected.
Conclusions:
- Pigmented bacilli are ubiquitous and possess novel carotenoid biosynthetic pathways.
- Carotenoid production enhances UV resistance in Bacillus spores.
- These findings suggest potential industrial relevance for novel carotenoids and biosynthetic pathways.
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