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Related Experiment Video

Updated: Jun 19, 2026

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Published on: May 16, 2017

Carotenoids found in Bacillus.

R Khaneja¹, L Perez-Fons, S Fakhry

¹School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, UK.

Journal of Applied Microbiology

|November 3, 2009

Summary

This summary is machine-generated.

Pigmented aerobic spore-forming bacteria, primarily Bacillus species, are widespread in the environment. These bacteria produce yellow, orange, and pink carotenoids, offering enhanced UV protection and potential industrial applications.

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Identification of Fatty Acids in Bacillus cereus

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Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

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Identification of Fatty Acids in Bacillus cereus

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Published on: December 5, 2016

Area of Science:

Microbiology
Biochemistry
Environmental Science

Background:

Pigmented bacteria play roles in various ecosystems.
Spore-forming bacteria, like Bacillus, are known for their resilience.
Understanding pigment diversity and function is crucial.

Purpose of the Study:

To explore the variety of pigmented aerobic spore formers in diverse environments.
To determine the chemical composition of their pigments.
To investigate the biosynthetic pathways and functional significance of these pigments.

Main Methods:

Environmental sampling (soil, seawater, gastrointestinal tract).
Phylogenetic analysis using 16S rRNA sequencing for species identification.
Pigment analysis via High-Performance Liquid Chromatography-Photo Diode Array (HPLC-PDA).

Main Results:

Commonly identified pigments were yellow, orange, and pink carotenoids.
Isolates were predominantly Bacillus species, including known species like B. marisflavi and B. safensis.
Three main carotenoids with distinct absorption maxima were identified, suggesting a C30 biosynthetic pathway. A water-soluble carotenoid was also detected.

Conclusions:

Pigmented bacilli are ubiquitous and possess novel carotenoid biosynthetic pathways.
Carotenoid production enhances UV resistance in Bacillus spores.
These findings suggest potential industrial relevance for novel carotenoids and biosynthetic pathways.