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Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion
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Diffusion tensor imaging to assess axonal regeneration in peripheral nerves.

Helmar C Lehmann1, Jiangyang Zhang, Susumu Mori

  • 1Department of Neurology, Johns Hopkins University, Baltimore, MD, USA.

Experimental Neurology
|November 3, 2009
PubMed
Summary

Diffusion tensor imaging (DTI) shows promise for monitoring nerve regeneration. This magnetic resonance technique accurately reflects axon regeneration in mouse models, aiding therapeutic development.

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Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Medical Imaging

Background:

  • Developing reliable outcome measures for nerve regeneration is crucial for evaluating therapeutic interventions.
  • Diffusion Tensor Imaging (DTI) offers a non-invasive method to visualize nerve tracts and assess nerve fiber integrity.
  • Serial DTI application in living subjects could enable longitudinal monitoring of nerve regeneration.

Purpose of the Study:

  • To evaluate the efficacy of ex vivo high-resolution DTI in imaging intact and regenerating peripheral nerves in mice.
  • To correlate DTI findings with electrophysiology and histology for validation.
  • To assess DTI's sensitivity in detecting and quantifying axon regeneration.

Main Methods:

  • High-resolution DTI was performed on mouse sciatic nerves subjected to crush injury or complete transection.
  • DTI metrics, including fractional anisotropy (FA) and diffusivity, were measured in injured and uninjured nerve segments.
  • DTI data were correlated with morphometric analysis from histology.

Main Results:

  • DTI revealed significantly lower FA values in completely denervated nerve segments compared to uninjured nerves.
  • FA values and parallel diffusivity demonstrated a positive correlation with the total number of regenerating axons.
  • Comparable patterns of axon regeneration were observed across different mouse strains.

Conclusions:

  • Ex vivo DTI is a sensitive imaging technique for assessing peripheral nerve regeneration in mouse models.
  • DTI metrics correlate well with histological measures of axon regeneration.
  • These findings support the further development of DTI for monitoring nerve regeneration in clinical settings.