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Updated: Jun 19, 2026

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow
Published on: October 17, 2025
Qualitatively distinct phenotypes in the design space of biochemical systems
Michael A Savageau1, Rick A Fasani
1Department of Biomedical Engineering, University of California, Davis, CA 95616-5294, USA. masavageau@ucdavis.edu
Abstract:
Although characterization of the genotype has undergone revolutionary advances as a result of the successful genome projects, the chasm between our understanding of a fully characterized gene sequence and the phenotypic repertoire of the organism is as broad and deep as it was in the pre-genomic era. There are two fundamental unsolved problems that provide the context for the challenges in relating genotype to phenotype. We address one of these and describe a generic method for constructing a system design space in which qualitatively distinct phenotypes can be identified and counted, their relative fitness analyzed and compared, and their tolerance to change measured.
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