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Related Experiment Videos

Gene expression profiling: decoding breast cancer.

Femke de Snoo1, Richard Bender, Annuska Glas

  • 1Department of Medical Affairs, Agendia BV, Science Park 406, Amsterdam, The Netherlands. femke.desnoo@agendia.com

Surgical Oncology
|November 3, 2009
PubMed
Summary
This summary is machine-generated.

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A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...

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Gene expression assays are now used in early breast cancer treatment. This review covers their development, validation, and clinical utility, highlighting MammaPrint, an FDA-cleared assay for breast cancer management.

Area of Science:

  • Oncology
  • Molecular Diagnostics

Background:

  • Gene expression assays are increasingly utilized in clinical settings for early breast cancer patient management.
  • The recent introduction of these assays into daily practice necessitates a review of their development and validation.

Purpose of the Study:

  • To review the development of gene expression arrays for early breast cancer.
  • To summarize the validation of commercially available gene expression assays.
  • To discuss the clinical utility of these assays in patient care.

Main Methods:

  • Literature review of gene expression assays for breast cancer.
  • Summary of validation data for commercially available assays.
  • Focus on MammaPrint, an FDA-cleared assay.

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Main Results:

  • Gene expression assays have evolved significantly for clinical application in early breast cancer.
  • Several commercially available assays have undergone validation, with varying degrees of evidence.
  • MammaPrint is highlighted as a key assay, being the first and only FDA-cleared for breast cancer management.

Conclusions:

  • Gene expression assays provide valuable information to aid in the care of early breast cancer patients.
  • The clinical integration of these assays is a recent development with ongoing impact.
  • MammaPrint represents a significant advancement in FDA-cleared diagnostic tools for breast cancer management.