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Updated: Jun 19, 2026

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry
Published on: June 10, 2025
Artificial control of subtype-specific platelet-derived growth factor-receptor signaling
Masayuki Isa1, Yusaku Ohta, Shigeyuki Namiki
1Department of Neurobiology, Graduate School of Medicine, The University of Tokyo, Japan.
Abstract:
Platelet-derived growth factor (PDGF) signaling controls various physiological functions via two receptor subtypes: PDGF receptor (PDGFR) alpha and PDGFRbeta. Nevertheless, our understanding of their roles is limited because of a lack of pharmacological tools to discriminate subtype-specific signaling. We developed a chimeric receptor by combining ligand-binding-domain truncated PDGFRbeta with anti-fluorescein single chain antibody, expecting the control of PDGFRbeta-specific signaling by oligomerized fluorescein as an artificial agonist. Results show that calcium mobilization, Cdc42 activation, and cell migration were elicited specifically by the artificial ligand in cells expressing the chimeric receptor. Our method is expected to be useful to understand the subtype-specific roles of PDGFRs in various cellular functions.
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