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Related Experiment Video

Updated: Jun 19, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Mouse Naïve CD4⁺ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Requirement for the basic helix-loop-helix transcription factor Dec2 in initial TH2 lineage commitment.

Xuexian O Yang¹, Pornpimon Angkasekwinai, Jinfang Zhu

¹Department of Immunology, MD Anderson Cancer Center, Houston, Texas, USA.

Nature Immunology

|November 3, 2009

Summary

The transcription factor Dec2 is crucial for T helper type 2 (T(H)2) cell differentiation. Dec2 deficiency impairs T(H)2 cell development and promotes key cytokine production, establishing a regulatory circuit for T(H)2 lineage commitment.

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Area of Science:

Immunology
Molecular Biology
Cell Biology

Background:

T helper type 2 (T(H)2) cell differentiation is critical for adaptive immunity.
The molecular mechanisms governing naive CD4(+) T cell commitment to the T(H)2 lineage remain incompletely understood.

Purpose of the Study:

To investigate the role of the transcription factor Dec2 in T(H)2 cell differentiation.
To elucidate the regulatory mechanisms involving Dec2 in T(H)2 lineage commitment.

Main Methods:

Analysis of Dec2 expression in T(H)2 cells.
Assessment of T(H)2 differentiation in Dec2-deficient mice (in vitro and in vivo).
Investigation of Dec2's transcriptional targets, including JunB and GATA-3.

Main Results:

Dec2 is selectively expressed in T(H)2 cells.
Dec2-deficient CD4(+) T cells exhibit impaired T(H)2 differentiation in asthma models and during parasitic antigen challenge.
Dec2 directly activates transcription of JunB and GATA-3, promoting IL-4, IL-5, and IL-13 expression.
A feed-forward regulatory loop exists where GATA-3 induces Dec2 expression.

Conclusions:

Dec2 is a key regulator of T(H)2 cell differentiation.
Dec2 plays a vital role in initiating T(H)2 responses, essential for immunity against parasites and in allergic diseases like asthma.
The identified Dec2-GATA-3 feed-forward circuit is fundamental for robust T(H)2 lineage commitment.