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Updated: Jun 19, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy
Published on: March 8, 2016
N-linked glycoprotein analysis using dual-extraction ultrahigh-performance liquid chromatography and electrospray
S O Siu1, Maggie P Y Lam, Edward Lau
1Department of Chemistry, The University of Hong Kong, Hong Kong, China.
Abstract:
Although reverse-phase liquid chromatography (RP-LC) is a common technique for peptide separation in shotgun proteomics and glycoproteomics, it often provides unsatisfactory results for the analysis of glycopeptides and glycans. This bias against glycopeptides makes it difficult to study glycoproteins. By coupling mass spectrometry (MS) with a combination of RP-LC and normal-phase (NP)-LC as an integrated front-end separation system, we demonstrate that effective identification and characterization of both peptides and glycopeptides mixtures, and their constituent glycan structures, can be achieved from a single sample injection event.
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