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Related Concept Videos

Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...

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Related Experiment Video

Updated: Jun 19, 2026

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
13:19

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

Cancer gene profiling in prostate cancer.

Adam Foye1, Phillip G Febbo

  • 1Departments of Medicine and Molecular Genetics and Microbiology, Duke Institute for Genome Science and Policy, Duke University, Durham, NC, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 3, 2009
PubMed
Summary

High-quality gene expression data from prostate cancer is crucial for understanding the disease. This chapter details methods for processing tumor samples, applicable to other cancers too.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Gene profiling and expression analysis significantly advance prostate cancer research.
  • Generating high-quality expression data from prostate cancer samples is challenging.
  • Established methods for sample processing have been developed over the last nine years.

Purpose of the Study:

  • To present detailed techniques for processing prostate cancer specimens for gene profiling.
  • To provide extensive technical notes for macrodissection and laser capture microdissection methods.
  • To offer generalizable processes applicable to other tumor types.

Main Methods:

  • Detailed description of en bloc (macrodissection) techniques for sample processing.
  • In-depth explanation of laser capture microdissection for precise sample isolation.

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miRNA Expression Analyses in Prostate Cancer Clinical Tissues
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miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
12:13

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

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Last Updated: Jun 19, 2026

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
13:19

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
12:13

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

  • Discussion of specific methods utilized in the authors' laboratory.

    • Main Results:

    • Comprehensive protocols for preparing prostate cancer tissues for gene expression analysis.
    • Technical insights to ensure the generation of high-quality expression data.
    • Demonstration of the adaptability of these methods for various tumor types.

    Conclusions:

    • The presented techniques enable high-quality gene expression analysis in prostate cancer.
    • These methods are robust, generalizable to other cancers, and adaptable to different instruments or kits.
    • This work provides valuable protocols for researchers in oncology and molecular biology.