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Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)
Published on: October 30, 2016
Binary genomotyping using lipooligosaccharide biosynthesis genes distinguishes between Campylobacter jejuni isolates
G S Hotter1, I H Li, N P French
1AgResearch, Hopkirk Research Institute, Grasslands Research Centre, Palmerston North, New Zealand.
Epidemiology and Infection
|November 4, 2009
Summary
Differences in Campylobacter jejuni lipooligosaccharide (LOS) gene complements can create distinct genomotypes. This study reveals intra-ST mosaicism, aiding in tracing bacterial sources like poultry or wild birds.
Area of Science:
- Microbiology
- Genomics
- Epidemiology
Background:
- Campylobacter jejuni is a primary cause of bacterial gastroenteritis globally.
- Understanding C. jejuni genetic diversity is crucial for tracking outbreaks and sources.
Purpose of the Study:
- To determine if variations in the lipooligosaccharide (LOS) biosynthesis locus can define epidemiologically useful binary genomotypes in C. jejuni.
- To correlate LOS locus class and binary genomotype with multilocus sequence types (STs) in poultry-associated isolates.
Main Methods:
- Utilized a PCR-based approach to analyze gene complement within the LOS biosynthesis locus.
- Examined 87 C. jejuni isolates from poultry-associated STs collected during sentinel surveillance.
- Correlated LOS locus class and binary genomotype assignments with specific STs.
Main Results:
- Identified mosaicism in gene complement within specific STs (45, 190, 354, 474) at the intra-ST level.
- Demonstrated that binary genomotypes could suggest origins (e.g., poultry vs. wild bird) for individual ST-45 isolates.
- Observed broader congruence in LOS locus class and gene complement that differentiated between isolates from different STs.
Conclusions:
- Lipooligosaccharide (LOS) gene complement variations provide epidemiologically relevant genomotypes for Campylobacter jejuni.
- Intra-ST mosaicism in LOS biosynthesis genes exists, but conserved patterns also distinguish between different sequence types.
- This genetic information aids in tracing the sources of C. jejuni infections.
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