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Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
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Published on: November 10, 2017

Development of responsive lanthanide probes for cellular applications.

Elizabeth J New1, David Parker, David G Smith

  • 1Department of Chemistry, Durham University, South Road, Durham DH1 3LE, UK.

Current Opinion in Chemical Biology
|November 4, 2009
PubMed
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Developing targeted luminescent lanthanide probes is crucial for observing intracellular changes in metal ions, oxyanions, and pH within specific organelles. Careful design ensures minimal disruption to cellular functions like viability and proliferation.

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Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging
13:21

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

Published on: July 21, 2011

Area of Science:

  • Biochemistry
  • Cell Biology
  • Chemical Biology

Background:

  • Intracellular environment monitoring requires probes for bioactive species like metal ions, oxyanions, and pH.
  • Organelle-specific targeting (mitochondria, nucleus, lysosomes) is essential for localized intracellular analysis.
  • Luminescent lanthanide complexes offer potential for such sensing applications.

Purpose of the Study:

  • To define critical design features for luminescent lanthanide probes.
  • To review sub-cellular localization profiles of existing probes.
  • To emphasize minimizing perturbation of cellular homeostasis during probe development.

Main Methods:

  • Defining critical design features for luminescent lanthanide complexes.
  • Reviewing published sub-cellular localization data.
  • Evaluating cell uptake mechanisms (e.g., macropinocytosis).

Main Results:

  • Key design principles for effective luminescent lanthanide probes were identified.
  • Sub-cellular localization data for various probes were compiled and analyzed.
  • Macropinocytosis confirmed as a viable cell uptake pathway for many probes.

Conclusions:

  • Optimized luminescent lanthanide probes are vital tools for intracellular sensing.
  • Targeted probes must be designed to maintain cellular homeostasis.
  • Successful probe development requires consideration of probe design, localization, and cellular impact.